It is a well-established method in many areas that focus on. The MarketWatch News Department was not involved in the creation of this content. The ZetaPlus Analyzer relies on dynamic light scattering to measure the.
Small molecule aggregation analysis should be done at working concentrations in and working solvents (e.g. Dynamic light scattering (DLS) actually measures the translational diffusion of particles due to Brownian motion. Dynamic light scattering (DLS) is an optical analysis method for particle sizes in solutions. Nanoscience: Dynamic Light Scattering (DLS). The minimum amount of sample required depends on the size of the molecule, as large molecules scatter more light than small molecules.įor most protein samples, concentrations of 0.2 mg/ml or higher are recommended.Īs little as 0.125 mg/ml of lysozyme or 12.5 μg/ml of IgG can be detected. Samples should be filtered or centrifuged (6000g, 10-30 min) prior to transfer to the clear bottom plate to remove large aggregates and precipitates. Significant aggregates will make accurate size measurements difficult. 
DLS is very sensitive to small amounts of aggregates. Measures particle size and particle-size distribution based on the dynamic light-scattering method, and absolute molecular weight, radius of gyration, and the.
Detergents frequently have micelle size similar to that of proteins and scatter significantly.DLS is compatible with a range of buffers.
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